prk7 ha s6k1  (Addgene inc)

Journal: bioRxiv

Article Title: Female resistance to the metabolic benefits of protein restriction is reversed by ovariectomy in mice

doi: 10.64898/2026.03.31.715667

Figure Lengend Snippet: (A-G) mTOR signaling in the liver was determined by Western blot analysis of the following phosphorylated and unphosphorylated proteins; S6 (B), 4e-BP1 (C), S6K1 (D), eiF2α (E), AKT (F-G), HSP90 (loading control). (H-J) mTORC1 signaling in the muscle was determined by Western blot analysis of phosphorylated S6 (I), phosphorylated 4e-BP1 (K). HSP90 was used as a loading control. (B-G, I-J) Quantification was determined by normalizing phosphorylated protein to normal protein. (A-J) n=5-6 mice/group, quantification was determined using ImageJ by normalizing the phosphorylated protein to the unphosphorylated form. Three-way ANOVA between sex, diet, and gonadectomy with post hoc Šidák’s adjusted test for pairwise comparisons, *p<0.05. p values for the overall effect of sex, diet, and gonadectomy and the interactions represent the significant p values from the three-way ANOVA. Data are represented as mean ±SEM. Abbreviations: C, CTL (21% control protein), L, LP (7% low protein), Cast (castration), Fem (female), Ovx (ovariectomy), Gonad (gonadectomy). Created in BioRender. Knopf, B. (2026) https://BioRender.com/i0gripn

Article Snippet: Primary antibodies were used at 1:1,000 and were purchased from Cell Signaling Technology (Danvers, MA, USA): p-T389 p70 S6K1 (#9205), p70 S6K1 (#2708), p-Ser240/244 S6 (#2215), S6 (#2217), p-Thr37/46 4E-BP1(#2855), 4E-BP1 (#9452), p-S51 eiF2α (#3398), eiF2α (#5324), p- S473 AKT (#4060), p-T308 AKT (#9275), AKT (#4691), HSP90 (#4877).

Techniques: Western Blot, Control

Journal: PLOS One

Article Title: Myofibrillar protein accumulation but reduced protein synthesis in PDCD4-depleted myotubes

doi: 10.1371/journal.pone.0345305

Figure Lengend Snippet: L6 myotubes were treated as described in . Immunoblotting (A) and quantified data for p-AKT ser473 (B) , S6K1 thr389 (C) , S6 ser235/236 (D) and 4E-BP1 thr37/46 (E) are shown. Total proteins for some of the above signals are shown (F, G) . Myotubes in the two treatment groups were incubated in 1µM of puromycin for 30 minutes and sunset analysis was performed (H) . Data are presented as mean ± SEM, n = 3 independent experiments with 3 technical replicates per experiment, * p < 0.05.

Article Snippet: Antibodies to PDCD4 (#9535), p62 (#5114), beclin-1 (#3738), microtubule-associated proteins 1A/1B light chain 3B (LC3B) (#3868), phosphorylated (p) FoxO3a ser253 (#9466), p-AKT Ser473 (#4060), p-S6 Ser235/236 (#4858), and its kinase S6K1 thr389 (#9234) were purchased from Cell Signaling Technology (Danvers, MA, USA).

Techniques: Western Blot, Incubation